Tam Tam Basket, here we go again. This time, unlike four years ago, when they could not even be registered, the rules of the Italian Basketball Federation do not allow these guys to participate in the championship they deserved on the pitch. The company of Castelvolturno, province of Caserta, a splendid integration laboratory, would like to enroll in the under 17 and under 19 Excellence, the highest level of youth. A dream that hits the rule of fielding a maximum of two foreigners, for which the FIP is not granting exceptions. And according to the law, Tam Tam players are almost all. Children of immigrants, or second generation immigrants, who were born in Italy and attend Italian school.
?
Massimo Antonelli, a 68-year-old Roman former basketball champion with Naples and Virtus Bologna, brought them together. It did so in a symbolic place for inclusion given that the Municipality of Castelvolturno is the one with the highest incidence of non-EU inhabitants, especially Africans. A harsh context that exposes the youngest, and not only, to marginalization, drug trafficking and organized crime. In the gym of Pineta Grande for a few hours a week they escape from all this, even if the difficulties have never been lacking. “Ours is a never-ending story. Every time we seem to have reached a goal, a new obstacle arrives” wrote the club to explain the impossibility of taking part in the championship of Excellence, the one of national importance. A déjà-vu that takes us back to 2017, when Tam Tam was not allowed to play official matches. Then the company inspired a law (the “Salva Tam Tam” in fact), an amendment to ensure that the Federation would allow the registration of players without Italian citizenship. A provision that is valid only for the regional championships and that for two years – after the victory of the regional under 15 – has forbidden Antonelli’s team from competing at the highest level.
?
Tam Tam Basket is trying in every way to persuade the Fip to grant an exemption to a limit, that of the two foreigners, which represents a barrier to the “purchases” of the richest companies. In these days the boys have sent a letter to the federal number one Gianni Petrucci: “Dear President, we wish to enroll in the championship of Excellence: it can be, and we hope it will be, a very good opportunity to grow and get involved and show how much we are worth”. “We, of course – continue the youngsters – are aware of the rules that the Fip has set for the championships, but it is precisely for these rules that teams and groups like ours are unable to play and express their joy and freedom by doing one of the things. they love most, playing basketball “.
?
Massimo Antonelli, the soul of this “laboratory” of social integration, also pointed out the paradox: “Our kids inspired the ‘rule saves Tam Tam Basket’ and they are the first not to benefit from it”. “I remember – continues his outburst on social media – that they were all born in Italy, but children of foreign citizens, therefore also foreign citizens, and normally attend schools, many of them are also enrolled in the fifth year with the Fip”. He says that the Federation had been asked for an exemption: “They said no, explaining that they had also consulted the other teams with whom we should have played”. “Fortunately – he concludes – not all sports federations start to check which parents the children were born from. In fact there are federations that implement sport ius soli, more sensitive and more inclusive federations, which know how to keep up with the times, beware to social changes and who are able to seize the opportunities in the best possible way “. Meanwhile, against the exclusion of the under 17 from the category of Excellence, Tam Tam has resorted to the TAR.
October 28 – 5:04 pm
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We wish to give thanks to the author of this write-up for this remarkable web content
Tam Tam, no championship of Excellence for “Italian” foreigners: “From the Fip no to the derogation”
Tam Tam Basket, here we go again. This time, unlike four years ago, when they could not even be registered, the rules of the Italian Basketball Federation do not allow these guys to participate in the championship they deserved on the pitch. The company of Castelvolturno, province of Caserta, a splendid integration laboratory, would like to enroll in the under 17 and under 19 Excellence, the highest level of youth. A dream that hits the rule of fielding a maximum of two foreigners, for which the FIP is not granting exceptions. And according to the law, Tam Tam players are almost all. Children of immigrants, or second generation immigrants, who were born in Italy and attend Italian school.
?
Massimo Antonelli, a 68-year-old Roman former basketball champion with Naples and Virtus Bologna, brought them together. It did so in a symbolic place for inclusion given that the Municipality of Castelvolturno is the one with the highest incidence of non-EU inhabitants, especially Africans. A harsh context that exposes the youngest, and not only, to marginalization, drug trafficking and organized crime. In the gym of Pineta Grande for a few hours a week they escape from all this, even if the difficulties have never been lacking. “Ours is a never-ending story. Every time we seem to have reached a goal, a new obstacle arrives” wrote the club to explain the impossibility of taking part in the championship of Excellence, the one of national importance. A déjà-vu that takes us back to 2017, when Tam Tam was not allowed to play official matches. Then the company inspired a law (the “Salva Tam Tam” in fact), an amendment to ensure that the Federation would allow the registration of players without Italian citizenship. A provision that is valid only for the regional championships and that for two years – after the victory of the regional under 15 – has forbidden Antonelli’s team from competing at the highest level.
?
Tam Tam Basket is trying in every way to persuade the Fip to grant an exemption to a limit, that of the two foreigners, which represents a barrier to the “purchases” of the richest companies. In these days the boys have sent a letter to the federal number one Gianni Petrucci: “Dear President, we wish to enroll in the championship of Excellence: it can be, and we hope it will be, a very good opportunity to grow and get involved and show how much we are worth”. “We, of course – continue the youngsters – are aware of the rules that the Fip has set for the championships, but it is precisely for these rules that teams and groups like ours are unable to play and express their joy and freedom by doing one of the things. they love most, playing basketball “.
?
Massimo Antonelli, the soul of this “laboratory” of social integration, also pointed out the paradox: “Our kids inspired the ‘rule saves Tam Tam Basket’ and they are the first not to benefit from it”. “I remember – continues his outburst on social media – that they were all born in Italy, but children of foreign citizens, therefore also foreign citizens, and normally attend schools, many of them are also enrolled in the fifth year with the Fip”. He says that the Federation had been asked for an exemption: “They said no, explaining that they had also consulted the other teams with whom we should have played”. “Fortunately – he concludes – not all sports federations start to check which parents the children were born from. In fact there are federations that implement sport ius soli, more sensitive and more inclusive federations, which know how to keep up with the times, beware to social changes and who are able to seize the opportunities in the best possible way “. Meanwhile, against the exclusion of the under 17 from the category of Excellence, Tam Tam has resorted to the TAR.
October 28 – 5:04 pm
© REPRODUCTION RESERVED
We wish to give thanks to the author of this write-up for this remarkable web content
Tam Tam, no championship of Excellence for “Italian” foreigners: “From the Fip no to the derogation”
Until the last lap, the last hole, the last putt. Ross McGowan, 38, 204 races and few satisfactions, won. He did it in his own way, in an ingenious and unruly way at the end of a head-to-head that started from afar with Laurie Canter, both South English. A battle already consummated a year ago, but the Kpmg Trophy (Challenge Tour) title went to another. And today at some point it seemed to end the same way.
After the first nine holes of this sunny Sunday on Lake Garda, the only two players above par were just them, the leaders of the day before, who started tied at -19 and alone on the run for the victory of the 77th Open d ‘ Italy with three shots of the margin. Errors on both sides, constant exchanges of favors. While many came forward behind them, in the end they were always the ones to gamble. McGowan capable of everything from eagle to double bogey; from the provisional ball to the birdie in the same hole, the 16, pocketing from the bunker for a sudden slap to the opponent. On the other hand, all day long he had shown a regular game and nice irons on the green, but the ball never entered. Badly the putt, which instead the winner thanked at the end of each lap.
And once again on the 72nd hole this club paid off. McGowan holes from six meters for -20, Canter misses from three having to postpone his first title (-19). Same result already in the clubhouse for the Belgian veteran Nicolas Colsaerts, author of a good comeback that he hopes until the last in the playoff. Many tried it on the last lap. Sebastian Heisele (from Germany) stopped at -18 and split fifth position with – 17 Finnish Kristoffer Reitan (a great lap in 65), Dean Burmester (South African), Spaniard Adri Arnaus and Martin Kaymer, the German champion found this year, which has come close to the Italian title several times. It is Guido Migliozzi who does better than the seventeen blues who started and you got to the end. Crushes on the accelerator today with seven birdies, two bogeys and an eagle, for a final 65 and -13 that takes him to 22nd position. “I enjoyed it today, even though I missed four short putts,” he said.
Nice final lap and nice tournament by Federico Maccario (-12 and 30th position), ready to move up the category with a quality game in all departments: today he showed great skills in attacking the green, with decisive approaches and spectacular shots from the bunker . From Sanremo also the caddy and best friend Lapo, with whom he finds balance and smiles on the field. Same score for the Lapo player, Francesco Laporta from Puglia. “A good week with a bad hole, the 15th, where I lost four strokes. Now a flight to Cyprus and then maybe to South Africa”. In the end, this last-minute Italian Open (awarded 45 days before the race) ended with its head held high. Chervò San Vigilio resorts and holes were liked, they signed the longest players (even if one of the shortest ones won), they presented perfect greens and stood up to attacks. After all, the final -20 aligns with the same score of Hennie Otto (Turin La Mandria 2014), and with that of Bernd Wiesberger (Olgiata 2019).
?
1. 268(-20)Ross McGowan (Ing), 66-64-67-71
2. 269(-19) Laurie Canter (Ing), 6o-68-69-72; Nicolas Colsaerts (Bel), 68-67-66-68
4. 270 (-18) Sebastian Heisele (Ger) 68-67-66-69
5. 271(-17) Adri Arnaus (Spa), 65-70-67-69; Dean Burmester (SA), 64-68-68-71; Jonathan Caldwell (N. Irl) 67-68-68-68; Martin Kaymer (Ger) 69-66-68-68; Kristoffer Reitan (Fin) 66-70-70-65
?
22. 275 (-13) Guido Migliozzi (68-69-73-65)
30. 276(-12) Federico Maccario (70-70-68-68) – Francesco Laporta (68-70-69-69)
55. 281 (-7) Lorenzo Gagli (70-69-72-70)
63. 283(-5) Lorenzo Scalise (67-69-77-70)
65. 284 (-4) Renato Paratore (71-69-74-70)
25 October 2020 (change October 25, 2020 | 18:33)
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We wish to give thanks to the author of this write-up for this incredible web content
Golf, Open d’Italia: vince Ross McGowan
Until the last lap, the last hole, the last putt. Ross McGowan, 38, 204 races and few satisfactions, won. He did it in his own way, in an ingenious and unruly way at the end of a head-to-head that started from afar with Laurie Canter, both South English. A battle already consummated a year ago, but the Kpmg Trophy (Challenge Tour) title went to another. And today at some point it seemed to end the same way.
After the first nine holes of this sunny Sunday on Lake Garda, the only two players above par were just them, the leaders of the day before, who started tied at -19 and alone on the run for the victory of the 77th Open d ‘ Italy with three shots of the margin. Errors on both sides, constant exchanges of favors. While many came forward behind them, in the end they were always the ones to gamble. McGowan capable of everything from eagle to double bogey; from the provisional ball to the birdie in the same hole, the 16, pocketing from the bunker for a sudden slap to the opponent. On the other hand, all day long he had shown a regular game and nice irons on the green, but the ball never entered. Badly the putt, which instead the winner thanked at the end of each lap.
And once again on the 72nd hole this club paid off. McGowan holes from six meters for -20, Canter misses from three having to postpone his first title (-19). Same result already in the clubhouse for the Belgian veteran Nicolas Colsaerts, author of a good comeback that he hopes until the last in the playoff. Many tried it on the last lap. Sebastian Heisele (from Germany) stopped at -18 and split fifth position with – 17 Finnish Kristoffer Reitan (a great lap in 65), Dean Burmester (South African), Spaniard Adri Arnaus and Martin Kaymer, the German champion found this year, which has come close to the Italian title several times. It is Guido Migliozzi who does better than the seventeen blues who started and you got to the end. Crushes on the accelerator today with seven birdies, two bogeys and an eagle, for a final 65 and -13 that takes him to 22nd position. “I enjoyed it today, even though I missed four short putts,” he said.
Nice final lap and nice tournament by Federico Maccario (-12 and 30th position), ready to move up the category with a quality game in all departments: today he showed great skills in attacking the green, with decisive approaches and spectacular shots from the bunker . From Sanremo also the caddy and best friend Lapo, with whom he finds balance and smiles on the field. Same score for the Lapo player, Francesco Laporta from Puglia. “A good week with a bad hole, the 15th, where I lost four strokes. Now a flight to Cyprus and then maybe to South Africa”. In the end, this last-minute Italian Open (awarded 45 days before the race) ended with its head held high. Chervò San Vigilio resorts and holes were liked, they signed the longest players (even if one of the shortest ones won), they presented perfect greens and stood up to attacks. After all, the final -20 aligns with the same score of Hennie Otto (Turin La Mandria 2014), and with that of Bernd Wiesberger (Olgiata 2019).
?
1. 268(-20)Ross McGowan (Ing), 66-64-67-71
2. 269(-19) Laurie Canter (Ing), 6o-68-69-72; Nicolas Colsaerts (Bel), 68-67-66-68
4. 270 (-18) Sebastian Heisele (Ger) 68-67-66-69
5. 271(-17) Adri Arnaus (Spa), 65-70-67-69; Dean Burmester (SA), 64-68-68-71; Jonathan Caldwell (N. Irl) 67-68-68-68; Martin Kaymer (Ger) 69-66-68-68; Kristoffer Reitan (Fin) 66-70-70-65
?
22. 275 (-13) Guido Migliozzi (68-69-73-65)
30. 276(-12) Federico Maccario (70-70-68-68) – Francesco Laporta (68-70-69-69)
55. 281 (-7) Lorenzo Gagli (70-69-72-70)
63. 283(-5) Lorenzo Scalise (67-69-77-70)
65. 284 (-4) Renato Paratore (71-69-74-70)
25 October 2020 (change October 25, 2020 | 18:33)
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We wish to give thanks to the author of this write-up for this incredible web content
Golf, Open d’Italia: vince Ross McGowan
Tampa does its duty. He jokes a little too much with fire in the final after dominating the Eagles, but still ends up winning 28-22 without too many worries in Philadelphia on Thursday Night that opens the sixth day of the NFL. The reigning champions climb 5-1 record this season by grinding almost 400 attack yards, the final score does not give the idea of ??the obvious actual gap. Bucs ahead 28-7, then they do some phenomena, fiddling beyond the lawful, but Brady closes the accounts by no longer returning the oval to his opponents, milking the clock until it reaches zero a short distance from the opponent’s end zone.
?
Tampa reiterates that it is a Super Bowl team, this time it also runs well with Fournette and Jones, but the secondary seems to be the victim of a curse: the Bucs also lose Sherman for a muscle injury, now they have out the three main cornerbacks plus the best safety, Winfield. Their further growth passes from the recovery of the injured, even Gronkowski is still in the pits, and from reducing the penalties: another 120 yds given this time to the opponents. Philadelphia is a modest team: it does not have a quarterback up to par, Hurts does not know how to pitch for these levels, a staff of coaches with everything to prove, and does not contain the game on the opponent’s ground. It risks becoming a long season, a Via Crucis for the Eagles, now a 2-4 record. Having given up Wentz to launch Hurts is a capital sin.
?
Immediate touchdown for Tampa: Brady shoots Howard for 2 yards and 6 points. Philadelphia replies: Hurts supplies the evergreen tight end Ertz for 5 yards and 7-7. The Bucs lose Sherman to hamstring injury. Then Brady finds Brown for 23 yards and the scoreboard 14-7. Fourth goal of the season for his favorite receiver. Then Dean intercepts Hurts and Fournette converts the steal into a touchdown. Philly has at least one gasp: Harris intercepts Brady at halftime, after 229 straight passes by the 44-year-old quarterback. Eagles without attack, unable to take advantage of the flaws of the secondary opponent: Hurts 5/14 for just 54 yards passing at the end of the first half. Tampa ahead 21-7, dominating. The Bucs seem to close their accounts in the middle of the 3rd quarter with the second touchdown on Fournette’s run: 28-7. Yet it is not over. Because Hurts turns a 4th down into a running touchdown. Then an Inexplicable 4th down played by Coach Arians and not converted by Tampa in the middle of the field allows Philly to march, and finally Sanders is seen running, up to the goal that reopens the game. Again by Hurts, a running back who plays quarterback, still on the ground. 28-22 guests after the conversion from 2 points. Tampa is suddenly under pressure, away, with the public entering the game after several grumbles. But Brady doesn’t tremble. Closes a crucial 3rd down relying on the usual Brown for a 27 yard combination. And then with experience he manages the time left on the clock and allows the Bucs to equal the best start to the season in franchise history.
October 15 – 07:31
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Brady shines, it’s Tampa show in Philadelphia
Tampa does its duty. He jokes a little too much with fire in the final after dominating the Eagles, but still ends up winning 28-22 without too many worries in Philadelphia on Thursday Night that opens the sixth day of the NFL. The reigning champions climb 5-1 record this season by grinding almost 400 attack yards, the final score does not give the idea of ??the obvious actual gap. Bucs ahead 28-7, then they do some phenomena, fiddling beyond the lawful, but Brady closes the accounts by no longer returning the oval to his opponents, milking the clock until it reaches zero a short distance from the opponent’s end zone.
?
Tampa reiterates that it is a Super Bowl team, this time it also runs well with Fournette and Jones, but the secondary seems to be the victim of a curse: the Bucs also lose Sherman for a muscle injury, now they have out the three main cornerbacks plus the best safety, Winfield. Their further growth passes from the recovery of the injured, even Gronkowski is still in the pits, and from reducing the penalties: another 120 yds given this time to the opponents. Philadelphia is a modest team: it does not have a quarterback up to par, Hurts does not know how to pitch for these levels, a staff of coaches with everything to prove, and does not contain the game on the opponent’s ground. It risks becoming a long season, a Via Crucis for the Eagles, now a 2-4 record. Having given up Wentz to launch Hurts is a capital sin.
?
Immediate touchdown for Tampa: Brady shoots Howard for 2 yards and 6 points. Philadelphia replies: Hurts supplies the evergreen tight end Ertz for 5 yards and 7-7. The Bucs lose Sherman to hamstring injury. Then Brady finds Brown for 23 yards and the scoreboard 14-7. Fourth goal of the season for his favorite receiver. Then Dean intercepts Hurts and Fournette converts the steal into a touchdown. Philly has at least one gasp: Harris intercepts Brady at halftime, after 229 straight passes by the 44-year-old quarterback. Eagles without attack, unable to take advantage of the flaws of the secondary opponent: Hurts 5/14 for just 54 yards passing at the end of the first half. Tampa ahead 21-7, dominating. The Bucs seem to close their accounts in the middle of the 3rd quarter with the second touchdown on Fournette’s run: 28-7. Yet it is not over. Because Hurts turns a 4th down into a running touchdown. Then an Inexplicable 4th down played by Coach Arians and not converted by Tampa in the middle of the field allows Philly to march, and finally Sanders is seen running, up to the goal that reopens the game. Again by Hurts, a running back who plays quarterback, still on the ground. 28-22 guests after the conversion from 2 points. Tampa is suddenly under pressure, away, with the public entering the game after several grumbles. But Brady doesn’t tremble. Closes a crucial 3rd down relying on the usual Brown for a 27 yard combination. And then with experience he manages the time left on the clock and allows the Bucs to equal the best start to the season in franchise history.
October 15 – 07:31
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We want to say thanks to the author of this short article for this remarkable content
Brady shines, it’s Tampa show in Philadelphia
Journal Reference:
Scientists demonstrated these real-time detection tools in plants and anticipate their use in animals, bacteria and fungi with diverse applications for biotechnology, biosecurity, bioenergy and agriculture. The team described the successful development of the UV system in Horticulture Research and their proof-of-principle demonstration in ACS Synthetic Biology.
CRISPR technologies have quickly become the primary tools of bioengineering, and new versions are continually in development. Identifying whether an organism has been modified by CRISPR technology was previously a complex and time-consuming process.
?Before this, the only way to tell if genome engineering occurred was to do a forensic analysis,? said Paul Abraham, a bioanalytical chemist and head of ORNL?s Secure Ecosystem Engineering and Design Science Focus Area. ?To be successful, you would need to know what the genome looked like before it was rewritten. We wanted to design a platform where we could proactively observe CRISPR activity.?
The research team developed an efficient self-detect solution that takes advantage of the way CRISPR works to trigger the technology to reveal itself. Under normal conditions, CRISPR works by connecting with a short RNA sequence, known as the guide RNA, as it leads CRISPR to a matching DNA sequence. When the target DNA is found, CRISPR modifies the DNA by acting like tiny molecular scissors to cut through one or both strands of DNA, depending on the type of CRISPR technology in use.
Abraham likens their method to an alarm system with two components: a biosensor guide RNA that redirects CRISPR activity and a reporter protein that flags the activity. Researchers encode the two components into an organism?s DNA to enable the monitoring system.
With the self-detect system in place, the biosensor guide RNA intercepts CRISPR, preventing CRISPR from connecting with its original gene target and redirecting CRISPR to a specific DNA sequence that encodes for a nonfunctioning green fluorescent protein, or GFP. When CRISPR edits the sequence, it flips a switch that produces functioning GFP, which creates a green glow signaling CRISPR?s presence.
Because a microscope is required to see the glow from GFP, the researchers improved on their original method by replacing GFP with a similar reporter protein, called eYGFPuv, that is visible under the type of ultraviolet light commonly known as a black light.
?Now we can see whether CRISPR is active in real time regardless of the size, shape and location of the organisms we?re evaluating,? Abraham said. ?This flexibility speeds the bioengineering process and extends the biosensors? use in laboratory and field applications.?
Since CRISPR must be tailored to each organism for effective use, knowing whether the CRISPR technology is working in a particular plant or microbe can accelerate progress toward goals such as developing drought-resistant bioenergy crops and engineering bacteria to efficiently convert plants into sustainable aviation fuels.
?These tools allow us to quickly identify positive transformants with desired genetic changes we are targeting,? said Carrie Eckert, lead for ORNL?s Synthetic Biology Group. ?We are easily able to see the variants versus those where modification did not occur.?
The biosensors also provide an effective method to know whether CRISPR is still active after the desired modifications have taken effect. ORNL plant synthetic biologist and co-author Xiaohan Yang compares CRISPR?s genome editing activity to a beneficial surgery but cautions that ?you don?t want the surgeon to leave the scissors behind? as continued CRISPR activity could have unintended effects.
Yang envisions biosensor applications that could test the progeny of modified plants, for instance, to verify that the gene editing machinery did not transfer to them. With this technology, it is possible to survey an entire field of crops.
The research team created specific biosensors to detect various CRISPR tools, including Cas9 nuclease, prime editor, base editor and CRISPRa. They demonstrated the sensors individually with each sensor signaling the presence of a particular type of CRISPR tool. Abraham also sees the potential to combine the biosensors into a version that would flag multiple gene editing technologies at once.
?We?ll continue to optimize these biosensors to improve the security of next-generation biotechnologies,? Abraham said.
Contributors to the paper titled ?Expanding the application of a UV-visible reporter for transient gene expressions and stable transformation in plants? include ORNL?s Guoliang Yuan, Haiwei Lu, Md. Mahmudul Hassan, Jin-Gui Chen, Gerald Tuskan and Xiaohan Yang, as well as Dan Tang and Yi Li of the University of Connecticut.
The scientists detailed their proof-of-principle demonstration in ACS Synthetic Biology. Co-authors on the paper, ?Plant-based biosensors for detecting CRISPR-mediated genome editing,? include ORNL?s Guoliang Yuan, Md. Mahmudul Hassan, Tao Yao, Haiwei Lu, Michael Vergara, Wellington Muchero, Jin-Gui Chen, Gerald Tuskan, Paul Abraham, Xiaohan Yang and Jesse Labbe (now at Invaio Sciences), as well as Changtian Pan and Yiping Qi of the University of Maryland.
The research was supported by the Center for Bioenergy Innovation and the Secure Ecosystem Engineering and Design Science Focus Area, which are both funded by the Biological and Environmental Research program in DOE?s Office of Science. Additional support was provided by the National Science Foundation.
Biosensors detect CRISPR activity, video: https://www.youtube.com/watch?v=mYvFyksphp8
We wish to give thanks to the author of this write-up for this incredible material
New biosensors shine a light on CRISPR gene editing
Journal Reference:
Scientists demonstrated these real-time detection tools in plants and anticipate their use in animals, bacteria and fungi with diverse applications for biotechnology, biosecurity, bioenergy and agriculture. The team described the successful development of the UV system in Horticulture Research and their proof-of-principle demonstration in ACS Synthetic Biology.
CRISPR technologies have quickly become the primary tools of bioengineering, and new versions are continually in development. Identifying whether an organism has been modified by CRISPR technology was previously a complex and time-consuming process.
?Before this, the only way to tell if genome engineering occurred was to do a forensic analysis,? said Paul Abraham, a bioanalytical chemist and head of ORNL?s Secure Ecosystem Engineering and Design Science Focus Area. ?To be successful, you would need to know what the genome looked like before it was rewritten. We wanted to design a platform where we could proactively observe CRISPR activity.?
The research team developed an efficient self-detect solution that takes advantage of the way CRISPR works to trigger the technology to reveal itself. Under normal conditions, CRISPR works by connecting with a short RNA sequence, known as the guide RNA, as it leads CRISPR to a matching DNA sequence. When the target DNA is found, CRISPR modifies the DNA by acting like tiny molecular scissors to cut through one or both strands of DNA, depending on the type of CRISPR technology in use.
Abraham likens their method to an alarm system with two components: a biosensor guide RNA that redirects CRISPR activity and a reporter protein that flags the activity. Researchers encode the two components into an organism?s DNA to enable the monitoring system.
With the self-detect system in place, the biosensor guide RNA intercepts CRISPR, preventing CRISPR from connecting with its original gene target and redirecting CRISPR to a specific DNA sequence that encodes for a nonfunctioning green fluorescent protein, or GFP. When CRISPR edits the sequence, it flips a switch that produces functioning GFP, which creates a green glow signaling CRISPR?s presence.
Because a microscope is required to see the glow from GFP, the researchers improved on their original method by replacing GFP with a similar reporter protein, called eYGFPuv, that is visible under the type of ultraviolet light commonly known as a black light.
?Now we can see whether CRISPR is active in real time regardless of the size, shape and location of the organisms we?re evaluating,? Abraham said. ?This flexibility speeds the bioengineering process and extends the biosensors? use in laboratory and field applications.?
Since CRISPR must be tailored to each organism for effective use, knowing whether the CRISPR technology is working in a particular plant or microbe can accelerate progress toward goals such as developing drought-resistant bioenergy crops and engineering bacteria to efficiently convert plants into sustainable aviation fuels.
?These tools allow us to quickly identify positive transformants with desired genetic changes we are targeting,? said Carrie Eckert, lead for ORNL?s Synthetic Biology Group. ?We are easily able to see the variants versus those where modification did not occur.?
The biosensors also provide an effective method to know whether CRISPR is still active after the desired modifications have taken effect. ORNL plant synthetic biologist and co-author Xiaohan Yang compares CRISPR?s genome editing activity to a beneficial surgery but cautions that ?you don?t want the surgeon to leave the scissors behind? as continued CRISPR activity could have unintended effects.
Yang envisions biosensor applications that could test the progeny of modified plants, for instance, to verify that the gene editing machinery did not transfer to them. With this technology, it is possible to survey an entire field of crops.
The research team created specific biosensors to detect various CRISPR tools, including Cas9 nuclease, prime editor, base editor and CRISPRa. They demonstrated the sensors individually with each sensor signaling the presence of a particular type of CRISPR tool. Abraham also sees the potential to combine the biosensors into a version that would flag multiple gene editing technologies at once.
?We?ll continue to optimize these biosensors to improve the security of next-generation biotechnologies,? Abraham said.
Contributors to the paper titled ?Expanding the application of a UV-visible reporter for transient gene expressions and stable transformation in plants? include ORNL?s Guoliang Yuan, Haiwei Lu, Md. Mahmudul Hassan, Jin-Gui Chen, Gerald Tuskan and Xiaohan Yang, as well as Dan Tang and Yi Li of the University of Connecticut.
The scientists detailed their proof-of-principle demonstration in ACS Synthetic Biology. Co-authors on the paper, ?Plant-based biosensors for detecting CRISPR-mediated genome editing,? include ORNL?s Guoliang Yuan, Md. Mahmudul Hassan, Tao Yao, Haiwei Lu, Michael Vergara, Wellington Muchero, Jin-Gui Chen, Gerald Tuskan, Paul Abraham, Xiaohan Yang and Jesse Labbe (now at Invaio Sciences), as well as Changtian Pan and Yiping Qi of the University of Maryland.
The research was supported by the Center for Bioenergy Innovation and the Secure Ecosystem Engineering and Design Science Focus Area, which are both funded by the Biological and Environmental Research program in DOE?s Office of Science. Additional support was provided by the National Science Foundation.
Biosensors detect CRISPR activity, video: https://www.youtube.com/watch?v=mYvFyksphp8
We wish to give thanks to the author of this write-up for this incredible material
New biosensors shine a light on CRISPR gene editing
After 17 consecutive years reaching at least the last 16 of the Champions League, Barcelona orphaned by Messi has slipped into the Europa League, a competition they last attended in 2003, the last pre-Messi year. The season started with Ronald Koeman and now continues with Xavi Hernandez. La Liga has already gone, Barça are -18 from Madrid, and the Catalans will have to fight to get to the Champions League.
?
Inserted in the group with Bayern Munich, Benfica and Dinamo Kiev, Barcelona managed to score two miserable golletti, both for the Ukrainian team coached by Mircea Lucescu in what were also the only victories of this European autumn: double 1-0. Then three 3-0, two with Bayern and one in Lisbon, and the key match: the 0-0 at home with Benfica which opened the door to the second round for the Portuguese.
?
Not immediate response. Koeman varied between 4-3-3 and 3-5-2, Xavi added 3-4-3. Sunday in the draw with Osasuna not even a pure full-back. If we consider that in February all the injured will be recovered (except Aguero, who this week should announce his retirement due to the arrhythmia problems recently encountered) with Pedri and Ansu Fati as key men, it is very likely to think about the confirmation of the 3-4- 3.
?
With Messi gone, the post remained vacant. It could have been Pedri, but after playing 68 games at the age of 18 in his first season, he continues to get hurt. The same goes for Ansu Fati, who returned after 10 months and immediately stopped by physical problems. We think of Ousmane Dembélé, but he is another crystal. So we choose Sergio Busquets, who acts as a hen for many young people and maintains a very very high level.
?
There is no shortage of them, and they are the only happy note for the Catalans in this troubled start to the season. First it was Gavi, born in August 2004 and finished straight for the national team. Then Nico, born in 2002, another permanent owner. The latest is Ezzalzouli, known as Abde, a Moroccan born in December 2001, taken from Hercules in the third series for Barça B and promoted to the first team. Excellent progression of Araujo in defense. The charrua is a veteran: he is from 1999.
?
After 6 years in Qatar as a player and then coach of Al Sadd Xavi Hernandez has returned home to try to save the club. So far, things have not gone very well for Guardiola’s former captain and symbol of Barça, who has won 25 trophies in 17 seasons playing 767 games at the Camp Nou on the pitch. The ideas are there, the squad is what it is and the difficulties are evident. Laporta hopes it is the new Guardiola, or at least the new Rijkaard, the road is complex.
?
(3-4-3) Ter Stegen; Araujo, Piqué, Eric Garcia; Gavi, Busquets, Pedri, Jordi Alba; Dembélé, Memphis Depay, Ansu Fati.
December 13, 2021 (change December 13, 2021 | 16:51)
© REPRODUCTION RESERVED
We would love to thank the author of this short article for this remarkable material
For Barcelona the cup is the last resort: Naples, here are the dangers
After 17 consecutive years reaching at least the last 16 of the Champions League, Barcelona orphaned by Messi has slipped into the Europa League, a competition they last attended in 2003, the last pre-Messi year. The season started with Ronald Koeman and now continues with Xavi Hernandez. La Liga has already gone, Barça are -18 from Madrid, and the Catalans will have to fight to get to the Champions League.
?
Inserted in the group with Bayern Munich, Benfica and Dinamo Kiev, Barcelona managed to score two miserable golletti, both for the Ukrainian team coached by Mircea Lucescu in what were also the only victories of this European autumn: double 1-0. Then three 3-0, two with Bayern and one in Lisbon, and the key match: the 0-0 at home with Benfica which opened the door to the second round for the Portuguese.
?
Not immediate response. Koeman varied between 4-3-3 and 3-5-2, Xavi added 3-4-3. Sunday in the draw with Osasuna not even a pure full-back. If we consider that in February all the injured will be recovered (except Aguero, who this week should announce his retirement due to the arrhythmia problems recently encountered) with Pedri and Ansu Fati as key men, it is very likely to think about the confirmation of the 3-4- 3.
?
With Messi gone, the post remained vacant. It could have been Pedri, but after playing 68 games at the age of 18 in his first season, he continues to get hurt. The same goes for Ansu Fati, who returned after 10 months and immediately stopped by physical problems. We think of Ousmane Dembélé, but he is another crystal. So we choose Sergio Busquets, who acts as a hen for many young people and maintains a very very high level.
?
There is no shortage of them, and they are the only happy note for the Catalans in this troubled start to the season. First it was Gavi, born in August 2004 and finished straight for the national team. Then Nico, born in 2002, another permanent owner. The latest is Ezzalzouli, known as Abde, a Moroccan born in December 2001, taken from Hercules in the third series for Barça B and promoted to the first team. Excellent progression of Araujo in defense. The charrua is a veteran: he is from 1999.
?
After 6 years in Qatar as a player and then coach of Al Sadd Xavi Hernandez has returned home to try to save the club. So far, things have not gone very well for Guardiola’s former captain and symbol of Barça, who has won 25 trophies in 17 seasons playing 767 games at the Camp Nou on the pitch. The ideas are there, the squad is what it is and the difficulties are evident. Laporta hopes it is the new Guardiola, or at least the new Rijkaard, the road is complex.
?
(3-4-3) Ter Stegen; Araujo, Piqué, Eric Garcia; Gavi, Busquets, Pedri, Jordi Alba; Dembélé, Memphis Depay, Ansu Fati.
December 13, 2021 (change December 13, 2021 | 16:51)
© REPRODUCTION RESERVED
We would love to thank the author of this short article for this remarkable material
For Barcelona the cup is the last resort: Naples, here are the dangers
Journal Reference:
The study published today in Nature Communications outlines the genes that are switched on or off in osteocytes, a type of bone cell that controls how other types of cells make or break down parts of the skeleton to maintain strong and healthy bones.
?This new information provides a kind of genetic shortlist we can look to when diagnosing bone diseases that have a genetic component,? says the study?s first author Dr Scott Youlten, Research Officer in the Bone Biology Lab. ?Identifying this unique genetic pattern will also help us find new therapies for bone disease and better understand the impacts of current therapies on the skeleton.?
A first look at the osteocyte transcriptome
The skeleton is a highly dynamic structure that changes shape and composition throughout a person?s life. Osteocytes are the most abundant cell type in bone but have proved difficult to study because they are embedded within the hard mineral structure of the skeleton.
Inside the bone, osteocytes form a network similar in scale and complexity to the neurons in the brain (with over 23 trillion connections between 42 billion osteocytes) that monitors bone health and responds to ageing and damage by signalling other cells to build more bone or break down old bone. Diseases such as osteoporosis and rare genetic skeletal disorders arise from an imbalance in these processes.
To understand what genes are involved in controlling bone build-up or breakdown, the researchers isolated bone samples from different skeletal sites of experimental models to measure the average gene activity in osteocytes. Through this, they mapped a comprehensive osteocyte ?signature? of 1239 genes that are switched on in osteocytes and that distinguish them from other cells. 77% of these genes have no previously known role in the skeleton and many were completely novel and only found in these critical cells.
?Many of the genes we saw enriched in osteocytes are also found in neurons, which is interesting given these cells share similar physical characteristics and may suggest they are more closely related than we previously thought,? explains Dr Youlten.
A comparison of the osteocyte signature genes with human genetic association studies of osteoporosis identify new genes that maybe associated with susceptibility to this common skeleton disease. Furthermore many of these osteocyte genes were also shown to cause rare bone diseases.
?Mapping the osteocyte transcriptome could help clinicians and researchers more easily establish whether a rare bone disease has a genetic cause, by looking through the ?shortlist? of genes known to play an active role in controlling the skeleton,? says Dr Youlten.
The big picture view
Co-senior author Professor Peter Croucher, Deputy Director of the Garvan Institute and Head of the Bone Biology Lab, says that ?the osteocyte transcriptome map gives researchers a picture of the whole landscape of genes that are switched on in osteocytes for the first time, rather than just a small glimpse.?
?The majority of genes that we?ve found to be active within osteocytes had no previously known role in bones. This discovery will help us understand what controls the skeleton, which genes are important in rare and common skeletal diseases and help us identify new treatments that can stop development of bone disease and also restore lost bone.?
This work was supported by a Wellcome Trust Strategic Award (101123/Z/13/A) and by Mrs Janice Gibson and the Ernest Heine Family Foundation.
This work is part of an international collaboration with researchers from University of Queensland, Imperial College London, The Wellcome Trust Sanger Institute, Cambridge, the Institute of Translational Genomics, Munich, King?s College London, Guy?s Hospital, London, the New York Genome Center, New York , Erasmus MC, Netherlands, and McGill University, Canada.
We want to thank the writer of this short article for this incredible web content
New map reveals genes that control the skeleton
Journal Reference:
The study published today in Nature Communications outlines the genes that are switched on or off in osteocytes, a type of bone cell that controls how other types of cells make or break down parts of the skeleton to maintain strong and healthy bones.
?This new information provides a kind of genetic shortlist we can look to when diagnosing bone diseases that have a genetic component,? says the study?s first author Dr Scott Youlten, Research Officer in the Bone Biology Lab. ?Identifying this unique genetic pattern will also help us find new therapies for bone disease and better understand the impacts of current therapies on the skeleton.?
A first look at the osteocyte transcriptome
The skeleton is a highly dynamic structure that changes shape and composition throughout a person?s life. Osteocytes are the most abundant cell type in bone but have proved difficult to study because they are embedded within the hard mineral structure of the skeleton.
Inside the bone, osteocytes form a network similar in scale and complexity to the neurons in the brain (with over 23 trillion connections between 42 billion osteocytes) that monitors bone health and responds to ageing and damage by signalling other cells to build more bone or break down old bone. Diseases such as osteoporosis and rare genetic skeletal disorders arise from an imbalance in these processes.
To understand what genes are involved in controlling bone build-up or breakdown, the researchers isolated bone samples from different skeletal sites of experimental models to measure the average gene activity in osteocytes. Through this, they mapped a comprehensive osteocyte ?signature? of 1239 genes that are switched on in osteocytes and that distinguish them from other cells. 77% of these genes have no previously known role in the skeleton and many were completely novel and only found in these critical cells.
?Many of the genes we saw enriched in osteocytes are also found in neurons, which is interesting given these cells share similar physical characteristics and may suggest they are more closely related than we previously thought,? explains Dr Youlten.
A comparison of the osteocyte signature genes with human genetic association studies of osteoporosis identify new genes that maybe associated with susceptibility to this common skeleton disease. Furthermore many of these osteocyte genes were also shown to cause rare bone diseases.
?Mapping the osteocyte transcriptome could help clinicians and researchers more easily establish whether a rare bone disease has a genetic cause, by looking through the ?shortlist? of genes known to play an active role in controlling the skeleton,? says Dr Youlten.
The big picture view
Co-senior author Professor Peter Croucher, Deputy Director of the Garvan Institute and Head of the Bone Biology Lab, says that ?the osteocyte transcriptome map gives researchers a picture of the whole landscape of genes that are switched on in osteocytes for the first time, rather than just a small glimpse.?
?The majority of genes that we?ve found to be active within osteocytes had no previously known role in bones. This discovery will help us understand what controls the skeleton, which genes are important in rare and common skeletal diseases and help us identify new treatments that can stop development of bone disease and also restore lost bone.?
This work was supported by a Wellcome Trust Strategic Award (101123/Z/13/A) and by Mrs Janice Gibson and the Ernest Heine Family Foundation.
This work is part of an international collaboration with researchers from University of Queensland, Imperial College London, The Wellcome Trust Sanger Institute, Cambridge, the Institute of Translational Genomics, Munich, King?s College London, Guy?s Hospital, London, the New York Genome Center, New York , Erasmus MC, Netherlands, and McGill University, Canada.
We want to thank the writer of this short article for this incredible web content
New map reveals genes that control the skeleton
Dominates. As if it weren’t two weeks away from his 37th birthday. As if this weren’t his 19th season in the NBA. As if what he experienced until a few days ago wasn’t the most complicated start to the season in his career. LeBron James dominates like a true king, an unleashed fury that strikes Orlando with 30 points, 11 rebounds and 10 assists. ?My secret? Sleeping – he says as soon as the game is over, with the ice pack on his back as a reminder of all the troubles he has had so far -. I slept from midnight to 8 and then from 8.30 to 12.30 before the game. And then there is the energy that my children give me ?. The Lakers (15 won-13 lost so far (they needed every drop of LeBron’s energy, who saved them with a third space quarter from the fool against Orlando (5-23), stretched 106-94.
?
James was devastating in the third period: 14 points, 3 assists, 2 blocks at LeBron, the kind that inflame the crowd and intimidate the opponents. After the troubles of the beginning of the season, King James finally seems to rise in condition: with the Magic second triple double of the season, sixth time in the last 8 races with at least 30 points. ?I’m improving every day – he says -: I always feel better, and this means being in the right place at the right time. And then we are getting to know each other better: the more we are on the pitch together the better, because we know where our teammates will be without even having to look “. The Lakers clung to the anchor LeBron, who in that quarter after the second half scored more points on his own than all the Magic, stopped at 10 with 2/23 from the field and driven down by 25 from a run of 23-0. “LeBron inspired us in that third quarter and we all played like him: with the maximum effort, regardless of the score and the opponent.” It didn’t last long, because the Magic had played better in the first half and in the fourth period they returned down to -10, with the Lakers in pain but kept safe by LeBron. In addition to James, Talen Horton-Tucker very well (19 points and 6 steals), precious Carmelo Anthony off the bench with 13 points, Russell Westbrook finished at 19 but still can’t find his place on the pitch, especially with LeBron so inspired . The absence of Anthony Davis, out for the second race in a row to treat his sore left knee, does not help to work on the understanding. The Lakers remain far from being a finished product, but at least now they have the LeBron certainty.
?
Orlando loses for the fifth game in a row and 12th in the last 13 games. The Magic are a sort of continuous audition for the many young people who are part of it, with the company desperate for certainties on which to build the future. One seems to be Cole Anthony, 21 points, 6 rebounds and 5 assists and star player along with Frantz Wagner (20 points and 7 rebounds) of the fourth quarter comeback. Mo Bamba’s injury, knocked out at the end of the third quarter due to a sprained right ankle, further crowded the infirmary. The Magic in the first half played better than the Lakers, and without the sensational blackout of the third period they probably would have played until the end. But for a young group that looks to tomorrow rather than today, even in a defeat like this there are important lessons.
?
Orlando almost always ahead in the first half, closed by the guests at 52-49. The third period is that of King James, the one in which LeBron rages, the Lakers defend and Orlando sinks basket after basket. The fourth period seems destined to be garbage time, but the Magic with Anthony and Wagner reopen the mach, bringing Orlando back to -10 three times. LeBron, however, gets the Lakers out of trouble and can enjoy another long night of sleep.
LA Lakers: James 30 (9/13 from two, 3/7 from three, 3/4 free throws), Westbrook 19, Horton-Tucker 19. Rebounds: James 11. Assists: James 10.
Orlando: Anthony 21 (2/7, 4/11, 5/6 tl), Wagner 20, Carter 16. Rimbalzi: Lopez 9. Assist: Anthony 5.
December 13, 2021 (change December 13, 2021 | 08:34)
© REPRODUCTION RESERVED
We want to give thanks to the author of this post for this remarkable content
LeBron saves the Lakers against Orlando: “My secret? I slept 12 hours”
Dominates. As if it weren’t two weeks away from his 37th birthday. As if this weren’t his 19th season in the NBA. As if what he experienced until a few days ago wasn’t the most complicated start to the season in his career. LeBron James dominates like a true king, an unleashed fury that strikes Orlando with 30 points, 11 rebounds and 10 assists. ?My secret? Sleeping – he says as soon as the game is over, with the ice pack on his back as a reminder of all the troubles he has had so far -. I slept from midnight to 8 and then from 8.30 to 12.30 before the game. And then there is the energy that my children give me ?. The Lakers (15 won-13 lost so far (they needed every drop of LeBron’s energy, who saved them with a third space quarter from the fool against Orlando (5-23), stretched 106-94.
?
James was devastating in the third period: 14 points, 3 assists, 2 blocks at LeBron, the kind that inflame the crowd and intimidate the opponents. After the troubles of the beginning of the season, King James finally seems to rise in condition: with the Magic second triple double of the season, sixth time in the last 8 races with at least 30 points. ?I’m improving every day – he says -: I always feel better, and this means being in the right place at the right time. And then we are getting to know each other better: the more we are on the pitch together the better, because we know where our teammates will be without even having to look “. The Lakers clung to the anchor LeBron, who in that quarter after the second half scored more points on his own than all the Magic, stopped at 10 with 2/23 from the field and driven down by 25 from a run of 23-0. “LeBron inspired us in that third quarter and we all played like him: with the maximum effort, regardless of the score and the opponent.” It didn’t last long, because the Magic had played better in the first half and in the fourth period they returned down to -10, with the Lakers in pain but kept safe by LeBron. In addition to James, Talen Horton-Tucker very well (19 points and 6 steals), precious Carmelo Anthony off the bench with 13 points, Russell Westbrook finished at 19 but still can’t find his place on the pitch, especially with LeBron so inspired . The absence of Anthony Davis, out for the second race in a row to treat his sore left knee, does not help to work on the understanding. The Lakers remain far from being a finished product, but at least now they have the LeBron certainty.
?
Orlando loses for the fifth game in a row and 12th in the last 13 games. The Magic are a sort of continuous audition for the many young people who are part of it, with the company desperate for certainties on which to build the future. One seems to be Cole Anthony, 21 points, 6 rebounds and 5 assists and star player along with Frantz Wagner (20 points and 7 rebounds) of the fourth quarter comeback. Mo Bamba’s injury, knocked out at the end of the third quarter due to a sprained right ankle, further crowded the infirmary. The Magic in the first half played better than the Lakers, and without the sensational blackout of the third period they probably would have played until the end. But for a young group that looks to tomorrow rather than today, even in a defeat like this there are important lessons.
?
Orlando almost always ahead in the first half, closed by the guests at 52-49. The third period is that of King James, the one in which LeBron rages, the Lakers defend and Orlando sinks basket after basket. The fourth period seems destined to be garbage time, but the Magic with Anthony and Wagner reopen the mach, bringing Orlando back to -10 three times. LeBron, however, gets the Lakers out of trouble and can enjoy another long night of sleep.
LA Lakers: James 30 (9/13 from two, 3/7 from three, 3/4 free throws), Westbrook 19, Horton-Tucker 19. Rebounds: James 11. Assists: James 10.
Orlando: Anthony 21 (2/7, 4/11, 5/6 tl), Wagner 20, Carter 16. Rimbalzi: Lopez 9. Assist: Anthony 5.
December 13, 2021 (change December 13, 2021 | 08:34)
© REPRODUCTION RESERVED
We want to give thanks to the author of this post for this remarkable content
LeBron saves the Lakers against Orlando: “My secret? I slept 12 hours”
The Peroni Top 10 rugby gives way to the national team of the championship, Italia Emergenti ex Italia A, for the last round of 2021. Saturday 18 December at 2 pm in Parma against Romania, the third in two months of this new route after Spain and Uruguay. To tell it, and to take stock of the tournament after the first 10 days, is Andrea Marcato. Coach of Petrarca Padova, unbeaten winter champion and assistant to coach Alessandro Troncon of the second national team (this week due to indisposition he will be replaced by Gonzalo Garcia).
What is your idea of ??this A / Emerging Italy?
“Compared to the one where I played as a player, it is much more organized and professional. It reminds me more of the senior national team than the slightly improvised ones of the time. There is the right pressure to hit the target of winning.”
“It’s an important stimulus. Before for the best Top 10 players there was no higher ambition. Or within a year you would move from Fir Academy to a Celtic franchise, otherwise you would stay there forever. Now with the Italy A / Emerging there is the superior ambition. I see him every week in training in Padua. This call-up includes a few players from the URC, none from the senior national team and the rest of the 10. I am happy that among the 30 called up there are 10 boys from Petrarca “.
The confirmation that Petrarca has the strongest squad in the league.
“We built it last season, in this one it was perfected with targeted insertions. For example Matteo Canali in the second row in place of Lorenzo Cannone who went to Treviso. He brought experience and leadership, learned playing in Rovigo alongside someone like Matteo Ferro. We have 51 players to manage between the first and second teams playing in Serie A “.
Petrarca dominated the first part of the season, like last year.
“Last year, due to Covid, we started playing late, there was an anomalous trend, for example we met Calvisano twice in the second leg. Now there is more continuity in the development of the team. We are happy to have overcoming the moment of many injuries, winning anyway against Calvisano, Rovigo and Colorno. The week we prepared this last match we had 24 players available out of 51 in the squad “
You are the favorites, after last season’s final and the excellent transfer market together with Valorugby. Do you feel the pressure?
“We have no pressure from the club. We want to do well, not take anyone lightly and, if we reach the final again, learn from the one lost in the last action against Rovigo. I always think about that match.”
Behind you, only the Valorugby seems to keep up.
“If we look at the transfer campaign and the history of the first round of the Top 10, we and Valorugby are stronger, also because we have changed less as technical staff”.
For the play-off race, only 5-a-side race, or the inclusion of the Lyons and Colorno revelations?
“For me it is a five-man race. The Fiamme Oro are the usual unknown, they have everything to do well, but they don’t necessarily have to. Calvisano has a good team, perhaps with a not so long squad. Rovigo has changed a lot and has a good potential to develop “.
Will it still be important to finish first?
“No, with the novelty of the final in the neutral first or quarter pitch, not much will change this year”.
?
Results of the 10th day:
FemiCz Rovigo-Mogliano 21-12, Viadana-Hbs Colorno 23-25,
postponed Fiamme Oro-Transvecta Calvisano, Sitav Lyons-Petrarca.
Classification: Petrarca * points 42; Valorugby Emilia * 37; Calvisano *, Colorno and Rovigo ** 25; Lyons * 21; Gold Flames *** 16; Viadana * 15; Mogliano ** 10; Lazio 5.
Next round, 29 January, 11th day: Lazio-Rovigo, Petrarca-Viadana, Calvisano-Mogliano, Valorugby-Lyons, Colorno-Fiamme Oro.
December 13, 2021 (change December 13, 2021 | 12:54)
© REPRODUCTION RESERVED
We would love to say thanks to the writer of this post for this outstanding web content
Marcato: “This Italy A is more organized. Petrarch favorite? We feel no pressure”
The Peroni Top 10 rugby gives way to the national team of the championship, Italia Emergenti ex Italia A, for the last round of 2021. Saturday 18 December at 2 pm in Parma against Romania, the third in two months of this new route after Spain and Uruguay. To tell it, and to take stock of the tournament after the first 10 days, is Andrea Marcato. Coach of Petrarca Padova, unbeaten winter champion and assistant to coach Alessandro Troncon of the second national team (this week due to indisposition he will be replaced by Gonzalo Garcia).
What is your idea of ??this A / Emerging Italy?
“Compared to the one where I played as a player, it is much more organized and professional. It reminds me more of the senior national team than the slightly improvised ones of the time. There is the right pressure to hit the target of winning.”
“It’s an important stimulus. Before for the best Top 10 players there was no higher ambition. Or within a year you would move from Fir Academy to a Celtic franchise, otherwise you would stay there forever. Now with the Italy A / Emerging there is the superior ambition. I see him every week in training in Padua. This call-up includes a few players from the URC, none from the senior national team and the rest of the 10. I am happy that among the 30 called up there are 10 boys from Petrarca “.
The confirmation that Petrarca has the strongest squad in the league.
“We built it last season, in this one it was perfected with targeted insertions. For example Matteo Canali in the second row in place of Lorenzo Cannone who went to Treviso. He brought experience and leadership, learned playing in Rovigo alongside someone like Matteo Ferro. We have 51 players to manage between the first and second teams playing in Serie A “.
Petrarca dominated the first part of the season, like last year.
“Last year, due to Covid, we started playing late, there was an anomalous trend, for example we met Calvisano twice in the second leg. Now there is more continuity in the development of the team. We are happy to have overcoming the moment of many injuries, winning anyway against Calvisano, Rovigo and Colorno. The week we prepared this last match we had 24 players available out of 51 in the squad “
You are the favorites, after last season’s final and the excellent transfer market together with Valorugby. Do you feel the pressure?
“We have no pressure from the club. We want to do well, not take anyone lightly and, if we reach the final again, learn from the one lost in the last action against Rovigo. I always think about that match.”
Behind you, only the Valorugby seems to keep up.
“If we look at the transfer campaign and the history of the first round of the Top 10, we and Valorugby are stronger, also because we have changed less as technical staff”.
For the play-off race, only 5-a-side race, or the inclusion of the Lyons and Colorno revelations?
“For me it is a five-man race. The Fiamme Oro are the usual unknown, they have everything to do well, but they don’t necessarily have to. Calvisano has a good team, perhaps with a not so long squad. Rovigo has changed a lot and has a good potential to develop “.
Will it still be important to finish first?
“No, with the novelty of the final in the neutral first or quarter pitch, not much will change this year”.
?
Results of the 10th day:
FemiCz Rovigo-Mogliano 21-12, Viadana-Hbs Colorno 23-25,
postponed Fiamme Oro-Transvecta Calvisano, Sitav Lyons-Petrarca.
Classification: Petrarca * points 42; Valorugby Emilia * 37; Calvisano *, Colorno and Rovigo ** 25; Lyons * 21; Gold Flames *** 16; Viadana * 15; Mogliano ** 10; Lazio 5.
Next round, 29 January, 11th day: Lazio-Rovigo, Petrarca-Viadana, Calvisano-Mogliano, Valorugby-Lyons, Colorno-Fiamme Oro.
December 13, 2021 (change December 13, 2021 | 12:54)
© REPRODUCTION RESERVED
We would love to say thanks to the writer of this post for this outstanding web content
Marcato: “This Italy A is more organized. Petrarch favorite? We feel no pressure”
@abuongi
Minus one: the countdown towards the start of the 4th Sport Festival is running out. Trento, from tomorrow to Sunday, is ready to host more than one hundred events with all its enthusiasm: the city is already colored in pink, in trembling expectation.
This year, among the stars of every discipline, the protagonists of athletics will be the masters. Thanks to what happened at the Tokyo Olympics at the beginning of August, an edition that, as a long wave of emotions, seems to never end. Trento will have the merit and the honor of bringing together the seven athletes who have won five historic gold medals in Japan. Yes, five. Two more than the maximum blue, which dated back to Moscow 1980 and Los Angeles 1984, the Games, however, halved by boycotts. In short: an Olympics like this, who knows when it will happen again.
?
To break the ice, Saturday at 16, at the Palazzo della Regione, with Drive to go crazy, will be Massimo Stano and Antonella Palmisano, the fantastic couple in Sapporo who dominated in the 24 hours of the 20 km. So much in common: the region of origin (Puglia), the coach (Patrizio Parcesepe), the training center (the sports center of the Yellow Flames in Castelporziano and the adjacent pine forest of Ostia). So much divides them, even temperamentally. As it turns out, between a joke and a tease. during the meeting in Trento. It’s guaranteed.
?
Following in turn, at 5 pm, at the Santa Chiara Auditorium, one of the most anticipated events: Two and a half meters above the sky. The Festival, on the occasion, will unite three of the greatest Olympic champions in the high jump. With two legends of the past to celebrate the phenomenon of the present. On the one hand, therefore, the American Dick Fosbury, the inventor of the style which, taking his name, is the only one used today at all levels, gold in Mexico City 1968, together with the Cuban Javier Sotomayor, with 2.45 record holder in the world of specialties for twenty-eight years and king in Barcelona 1992. On the other hand, Gianmarco Tamberi, two months ago triumphed in Tokyo together with his Qatari friend Mutaz Barshim with a script worthy of an Oscar film. The three, face to face, have never been there. And it is clear that, from such strong personalities, between anecdotes and technical disquisitions, a very sparkling encounter can only emerge.
?
On Sunday it will be the sprinters’ turn. Starting with ?The fastest man in the world?, Marcell Jacobs. The long-awaited crowd bath with the Gardesano, who has recently resumed training towards next season, is scheduled for 6 pm at the Santa Chiara Auditorium. The policeman will be accompanied by an exceptional guest, Valerij Borzov, the former Soviet now Ukrainian, bitter rival of Pietro Mennea, who at the 1972 Munich Games was gold in the 100 and 200. The two will meet again later the awards ceremony at the New Tokyo National Stadium. It was last August 2nd and Valerij, in the role of IOC member, handed Marcell his wonderful gold medal (topped off by the European record of 9 ?80). Immediately after (the scenario will not change), however Blue Lightning, together with Paolo Camossi’s pupil, on stage there will be the three companions of the 4×100 of wonders: Lorenzo Patta, Fausto Desalu and Filippo Tortu. It will be the ideal context to relive the joys of a final which, concluded by the spectacular comeback of Tortu in the last fraction, is already a legend and to clarify – if ever there was a need – the truth about the relationship between Marcell and Filippo. This relay, it is guaranteed, has a bright future ahead of it.
?
Not just Olympic champions. Or rather: not just today’s Olympic champions. At the Festival there will also be other meetings with athletics at the center. Like the one on Friday at 7.30pm at the Muse (Born to run), with Stefano Mei, current president of FIDAL and European gold of 10,000 in Stuttgart 1986, Alberto Cova, on the same distance triumphant at the European Championships in Athens 1982, at the World Championships in Helsinki 1983 and – indeed – at the 1984 Los Angeles Games and Yeman Crippa, the best blue middle-distance runner of the moment, Italian record holder of 3000, 5000 and 10,000. Finally on Saturday, at 10, always at the Muse, Elena Vallortigara, with her 2.02 the second tallest woman in Italy, will be among the protagonists of Sport after Covid.
October 6, 2021 (change October 7, 2021 | 17:23)
© REPRODUCTION RESERVED
We would love to give thanks to the writer of this write-up for this outstanding material
Jacobs, Tamberi, 4×100, Stano and Palmisano: a golden stage with Italian athletics
@abuongi
Minus one: the countdown towards the start of the 4th Sport Festival is running out. Trento, from tomorrow to Sunday, is ready to host more than one hundred events with all its enthusiasm: the city is already colored in pink, in trembling expectation.
This year, among the stars of every discipline, the protagonists of athletics will be the masters. Thanks to what happened at the Tokyo Olympics at the beginning of August, an edition that, as a long wave of emotions, seems to never end. Trento will have the merit and the honor of bringing together the seven athletes who have won five historic gold medals in Japan. Yes, five. Two more than the maximum blue, which dated back to Moscow 1980 and Los Angeles 1984, the Games, however, halved by boycotts. In short: an Olympics like this, who knows when it will happen again.
?
To break the ice, Saturday at 16, at the Palazzo della Regione, with Drive to go crazy, will be Massimo Stano and Antonella Palmisano, the fantastic couple in Sapporo who dominated in the 24 hours of the 20 km. So much in common: the region of origin (Puglia), the coach (Patrizio Parcesepe), the training center (the sports center of the Yellow Flames in Castelporziano and the adjacent pine forest of Ostia). So much divides them, even temperamentally. As it turns out, between a joke and a tease. during the meeting in Trento. It’s guaranteed.
?
Following in turn, at 5 pm, at the Santa Chiara Auditorium, one of the most anticipated events: Two and a half meters above the sky. The Festival, on the occasion, will unite three of the greatest Olympic champions in the high jump. With two legends of the past to celebrate the phenomenon of the present. On the one hand, therefore, the American Dick Fosbury, the inventor of the style which, taking his name, is the only one used today at all levels, gold in Mexico City 1968, together with the Cuban Javier Sotomayor, with 2.45 record holder in the world of specialties for twenty-eight years and king in Barcelona 1992. On the other hand, Gianmarco Tamberi, two months ago triumphed in Tokyo together with his Qatari friend Mutaz Barshim with a script worthy of an Oscar film. The three, face to face, have never been there. And it is clear that, from such strong personalities, between anecdotes and technical disquisitions, a very sparkling encounter can only emerge.
?
On Sunday it will be the sprinters’ turn. Starting with ?The fastest man in the world?, Marcell Jacobs. The long-awaited crowd bath with the Gardesano, who has recently resumed training towards next season, is scheduled for 6 pm at the Santa Chiara Auditorium. The policeman will be accompanied by an exceptional guest, Valerij Borzov, the former Soviet now Ukrainian, bitter rival of Pietro Mennea, who at the 1972 Munich Games was gold in the 100 and 200. The two will meet again later the awards ceremony at the New Tokyo National Stadium. It was last August 2nd and Valerij, in the role of IOC member, handed Marcell his wonderful gold medal (topped off by the European record of 9 ?80). Immediately after (the scenario will not change), however Blue Lightning, together with Paolo Camossi’s pupil, on stage there will be the three companions of the 4×100 of wonders: Lorenzo Patta, Fausto Desalu and Filippo Tortu. It will be the ideal context to relive the joys of a final which, concluded by the spectacular comeback of Tortu in the last fraction, is already a legend and to clarify – if ever there was a need – the truth about the relationship between Marcell and Filippo. This relay, it is guaranteed, has a bright future ahead of it.
?
Not just Olympic champions. Or rather: not just today’s Olympic champions. At the Festival there will also be other meetings with athletics at the center. Like the one on Friday at 7.30pm at the Muse (Born to run), with Stefano Mei, current president of FIDAL and European gold of 10,000 in Stuttgart 1986, Alberto Cova, on the same distance triumphant at the European Championships in Athens 1982, at the World Championships in Helsinki 1983 and – indeed – at the 1984 Los Angeles Games and Yeman Crippa, the best blue middle-distance runner of the moment, Italian record holder of 3000, 5000 and 10,000. Finally on Saturday, at 10, always at the Muse, Elena Vallortigara, with her 2.02 the second tallest woman in Italy, will be among the protagonists of Sport after Covid.
October 6, 2021 (change October 7, 2021 | 17:23)
© REPRODUCTION RESERVED
We would love to give thanks to the writer of this write-up for this outstanding material
Jacobs, Tamberi, 4×100, Stano and Palmisano: a golden stage with Italian athletics
Why are Facebook with Meta and Epic Games focusing so much on the metaverse? And what is the role of video games? The explanation gives us a leap in 2030 …
The year is 2030. You’re dining while your kids (adults who can’t start a family due to inflation) sit next to you, dabbing and wearing a VR helmet that feeds them spoonfuls of G-Fuel. Scroll your Twitter feed with an EyePhone, eye implants that render the internet in full augmented reality, and check out your video game library, now curated by a handful of huge companies that have incorporated all the smaller ones. Read a tweet and unlock an achievement for connecting for the first time in the day.
According to the tweet, Sonic is playable in Fortnite. It’s not a skin, it’s a whole game, the new Sonic Origins. Epic Games owns Sega in 2030, along with a handful of other huge mid-tier publishers and developers. Microsoft, Sony, Embracer Group, EA and Tencent all the others possess.
Out of pure curiosity, start Fortnite and find out what it has become come Ready Player One: an entirely digital world in which to play, meet friends, have fun? in one word, live as in an alternative reality. There is no need for a console, as it comes pre-installed on all TVs, eye implants and VR headsets. Head to Fortnite Plaza, a hub area where all your friends and family are enjoying their free time. Your wife is here, although she hates video games, because Epic has a deal with the big supermarkets where you can get a big discount available only for those who shop in the metaverse. He is strolling through the virtual shelves of a virtual store and filling a shopping cart, something Epic began to master when it implemented the shopping cart functionality in the Epic Games Store in 2025.
Hop into your Rocket League car and whiz around in search of Sonic, passing live gigs with real bouncers checking everyone’s tickets before entering – the metaverse is a place to play but it’s also the workplace for many. Shake your head and glide through Call of Duty Town, where people play the new Warzone map. To your left, another player tries to start a run with you, but he’s dressed as Cucumber Rick so go to the social menu and block him, removing him from your instance. A little further on is a help desk that is hiring admin to patrol Fortnite Plaza and make sure people are enjoying the product rather than being mean to others. Endless billboards they promote fast food and electric cars.
Finally, reach your destination. Sonic Origins, a new Sonic game within Fortnite. In reality, it’s less of a game and more of a minigame, but at least playing it earns you a backpack to wear in the mode Fortnite Battle Royale.
This is the future that video game companies have foreseen. An industry that is consolidating and in which everyone is chasing “The blue ocean”, a market strategy where competition is irrelevant because the rules of the game are still being decided. Everyone wants to be the first to build a metaverse, own more brands, and have as much power in their hands as possible over all the works of art that people care about. It’s not just Epic Games – almost everyone wants a slice.
Just this week, Epic Games announced that it has acquired Harmonix, the developer known for Rock Band. Harmonix “will collaborate closely with Epic to develop musical journeys and gameplay for Fortnite”, according to a note from Epic, which means that we will probably not see more Harmonix rhythm games: they will be only a part of Fortnite from now on. Epic Games also owns Psyonix, the creators of Rocket League, and Tonic Games Group, the home behind Fall Guys. And rest assured that others will follow.
?It’s no secret that Epic is interested in building the metaverse, and Tonic Games shares that goal,? Epic Games founder and CEO Tim Sweeney said at the time. “Epic works to build this virtual future, but we need great creative talent who know how to create powerful games, content and experiences.”
?Contents and experiences?: this is the important part of the declaration. This is the real plan. Epic Games will continue to incorporate studies to power everything Fortnite can do. Consolidation (the practice whereby large fish eat small fish, becoming bigger and bigger and, in fact, solid) is the enemy of diversity. The goal is make all games, everything, Fortnite. We’re already seeing commercials for Nike Jordan, John Wick, and Nfl popping up in the game, and we’re getting closer to a dystopian world that looks like it’s straight out of Blade Runner.
The fact is that in some ways we already live in the metaverse. Log into Twitter on your phone and you’re in another world full of people you probably won’t interact with once you’re out of it. Twitter has made people take jobs, get in trouble, and meet a partner. The web is already impacting our lives in real and tangible ways. The idea of ??metaverse video games is just another way to “entertain” people for money. In the last few years, ?Engagement? was a key metric for the success of video game companies. But was it a metric that did gamers good? No not at all.
The fact that players need to be constantly entertained in their video games is what has led to the creation of games as a service, battle passes and open world games where you play the game. same exact activity 5,000 times. It is what gave the loot boxes and all the similar mechanics, which have the sole objective of making us continue playing, and the metaverse is only an extension of it. And this path will lead to a situation where fewer smaller creators will be able to do the things they want to do, and more giant games and experiences that require all of your free time, luring you in with constant events and limited-time offers to keep you coming back.
This is not good for the developers either. Sure, consolidation helps some struggling teams stay afloat in the same way it does working externally, but do developers really want to work on the same project forever? It sounds difficult, and this will ultimately lead to creative burnout and the death of talent. After all, if you don’t like working in video games, why not try the much more lucrative route of software development? The events of recent times show a glimpse of the future, and what we have seen so far is certainly not exciting.
Written by Kirk McKeand for GLHF
© REPRODUCTION RESERVED
We would love to thank the author of this write-up for this amazing web content
Ready Player One is reality: the role of video games and why it is a problem
Why are Facebook with Meta and Epic Games focusing so much on the metaverse? And what is the role of video games? The explanation gives us a leap in 2030 …
The year is 2030. You’re dining while your kids (adults who can’t start a family due to inflation) sit next to you, dabbing and wearing a VR helmet that feeds them spoonfuls of G-Fuel. Scroll your Twitter feed with an EyePhone, eye implants that render the internet in full augmented reality, and check out your video game library, now curated by a handful of huge companies that have incorporated all the smaller ones. Read a tweet and unlock an achievement for connecting for the first time in the day.
According to the tweet, Sonic is playable in Fortnite. It’s not a skin, it’s a whole game, the new Sonic Origins. Epic Games owns Sega in 2030, along with a handful of other huge mid-tier publishers and developers. Microsoft, Sony, Embracer Group, EA and Tencent all the others possess.
Out of pure curiosity, start Fortnite and find out what it has become come Ready Player One: an entirely digital world in which to play, meet friends, have fun? in one word, live as in an alternative reality. There is no need for a console, as it comes pre-installed on all TVs, eye implants and VR headsets. Head to Fortnite Plaza, a hub area where all your friends and family are enjoying their free time. Your wife is here, although she hates video games, because Epic has a deal with the big supermarkets where you can get a big discount available only for those who shop in the metaverse. He is strolling through the virtual shelves of a virtual store and filling a shopping cart, something Epic began to master when it implemented the shopping cart functionality in the Epic Games Store in 2025.
Hop into your Rocket League car and whiz around in search of Sonic, passing live gigs with real bouncers checking everyone’s tickets before entering – the metaverse is a place to play but it’s also the workplace for many. Shake your head and glide through Call of Duty Town, where people play the new Warzone map. To your left, another player tries to start a run with you, but he’s dressed as Cucumber Rick so go to the social menu and block him, removing him from your instance. A little further on is a help desk that is hiring admin to patrol Fortnite Plaza and make sure people are enjoying the product rather than being mean to others. Endless billboards they promote fast food and electric cars.
Finally, reach your destination. Sonic Origins, a new Sonic game within Fortnite. In reality, it’s less of a game and more of a minigame, but at least playing it earns you a backpack to wear in the mode Fortnite Battle Royale.
This is the future that video game companies have foreseen. An industry that is consolidating and in which everyone is chasing “The blue ocean”, a market strategy where competition is irrelevant because the rules of the game are still being decided. Everyone wants to be the first to build a metaverse, own more brands, and have as much power in their hands as possible over all the works of art that people care about. It’s not just Epic Games – almost everyone wants a slice.
Just this week, Epic Games announced that it has acquired Harmonix, the developer known for Rock Band. Harmonix “will collaborate closely with Epic to develop musical journeys and gameplay for Fortnite”, according to a note from Epic, which means that we will probably not see more Harmonix rhythm games: they will be only a part of Fortnite from now on. Epic Games also owns Psyonix, the creators of Rocket League, and Tonic Games Group, the home behind Fall Guys. And rest assured that others will follow.
?It’s no secret that Epic is interested in building the metaverse, and Tonic Games shares that goal,? Epic Games founder and CEO Tim Sweeney said at the time. “Epic works to build this virtual future, but we need great creative talent who know how to create powerful games, content and experiences.”
?Contents and experiences?: this is the important part of the declaration. This is the real plan. Epic Games will continue to incorporate studies to power everything Fortnite can do. Consolidation (the practice whereby large fish eat small fish, becoming bigger and bigger and, in fact, solid) is the enemy of diversity. The goal is make all games, everything, Fortnite. We’re already seeing commercials for Nike Jordan, John Wick, and Nfl popping up in the game, and we’re getting closer to a dystopian world that looks like it’s straight out of Blade Runner.
The fact is that in some ways we already live in the metaverse. Log into Twitter on your phone and you’re in another world full of people you probably won’t interact with once you’re out of it. Twitter has made people take jobs, get in trouble, and meet a partner. The web is already impacting our lives in real and tangible ways. The idea of ??metaverse video games is just another way to “entertain” people for money. In the last few years, ?Engagement? was a key metric for the success of video game companies. But was it a metric that did gamers good? No not at all.
The fact that players need to be constantly entertained in their video games is what has led to the creation of games as a service, battle passes and open world games where you play the game. same exact activity 5,000 times. It is what gave the loot boxes and all the similar mechanics, which have the sole objective of making us continue playing, and the metaverse is only an extension of it. And this path will lead to a situation where fewer smaller creators will be able to do the things they want to do, and more giant games and experiences that require all of your free time, luring you in with constant events and limited-time offers to keep you coming back.
This is not good for the developers either. Sure, consolidation helps some struggling teams stay afloat in the same way it does working externally, but do developers really want to work on the same project forever? It sounds difficult, and this will ultimately lead to creative burnout and the death of talent. After all, if you don’t like working in video games, why not try the much more lucrative route of software development? The events of recent times show a glimpse of the future, and what we have seen so far is certainly not exciting.
Written by Kirk McKeand for GLHF
© REPRODUCTION RESERVED
We would love to thank the author of this write-up for this amazing web content
Ready Player One is reality: the role of video games and why it is a problem